PMID:12942368
| Citation |
Starcic-Erjavec, M, van Putten, JP, Gaastra, W, Jordi, BJ, Grabnar, M and Zgur-Bertok, D (2003) H-NS and Lrp serve as positive modulators of traJ expression from the Escherichia coli plasmid pRK100. Mol. Genet. Genomics 270:94-102 |
|---|---|
| Abstract |
Conjugative transfer of F-like plasmids is a tightly regulated process. The TraJ protein is the main positive activator of the tra operon which encodes products required for conjugative transfer of F-like plasmids. Nucleotide sequence analysis revealed potential Lrp and H-NS binding sites in the traJ regulatory region. Expression of a traJ-lacZ fusion in hns and lrp mutant strains showed that both are positive modulators of traJ expression. Competitive RT-PCR demonstrated that H-NS and Lrp exert their effect at the transcriptional level. Electrophoretic mobility-shift assays showed that H-NS and Lrp proteins bind to the traJ promoter. Conjugative transfer of pRK100 was decreased in hns but not in lrp mutant strains. Together, the results indicate H-NS and Lrp function as activators of traJ transcription. |
| Links |
PubMed Online version:10.1007/s00438-003-0908-1 |
| Keywords |
Bacterial Proteins/genetics; Base Sequence; Crosses, Genetic; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Gene Expression Regulation, Bacterial/genetics; Kinetics; Leucine-Responsive Regulatory Protein; Molecular Sequence Data; Plasmids/genetics; Proteins/genetics; Proteins/metabolism; RNA, Bacterial/genetics; RNA, Bacterial/isolation & purification; Recombinant Fusion Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; beta-Galactosidase/genetics |
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