PMID:12935896
| Citation |
Kato, Y, Nishiyama, K and Tokuda, H (2003) Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction. FEBS Lett. 550:114-8 |
|---|---|
| Abstract |
SecA and an apparatus comprising SecYEG and SecDF-YajC complexes catalyze protein translocation across the Escherichia coli membrane. SecDF-YajC and SecG facilitate membrane insertion of SecA, which is the driving force for protein translocation. Here we report that SecDF-YajC depletion together with SecG depletion nearly completely inhibits protein translocation both in vivo and in vitro, although SecDF-YajC had been thought to be unnecessary for in vitro translocation. The level of SecG in membranes decreased to about half upon SecDF-YajC depletion and recovered to a normal level when SecDF-YajC was expressed. SecDF-YajC inhibited disulfide bond formation between two SecG molecules possessing a single cysteine residue. These results suggest functional interaction between SecDF-YajC and SecG. |
| Links | |
| Keywords |
Antigens, Bacterial/genetics; Antigens, Bacterial/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Dimerization; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutation; Protein Transport; Transport Vesicles/metabolism |
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