PMID:12923096
| Citation |
Stewart, JB and Hermodson, MA (2003) Topology of RbsC, the membrane component of the Escherichia coli ribose transporter. J. Bacteriol. 185:5234-9 |
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| Abstract |
The topology of RbsC, the membrane component of the ribose transporter in Escherichia coli, has been determined by using 34 single-cysteine mutants and a modified fluorescence labeling technique designated multiplex labeling. This technique gives topology, expression, and localization information for a membrane protein from a single batch of bacterial cells. The results indicate that RbsC contains 10 transmembrane-spanning helices, with the N and C termini being in the cytosol. This topology matches predictions from the latest prediction programs and the topology of the similar, recently crystallized membrane protein BtuC. |
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| Keywords |
ATP-Binding Cassette Transporters/chemistry; ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Amino Acid Sequence; Amino Acid Substitution; Biological Transport; Cell Membrane/chemistry; Cell Membrane/metabolism; Cysteine; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fluorescein/metabolism; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Ribose/metabolism |
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