PMID:12903244
| Citation |
Asagoshi, K, Yamada, T, Terato, H, Ohyama, Y and Ide, H' (2000) Enzymatic properties of Escherichia coli and human 7,8-dihydro-8-oxoguanine DNA glycosylases. Nucleic Acids Symp. Ser. ':11-2 |
|---|---|
| Abstract |
Oxidative damage to DNA generates aberrant guanine bases such as 2,6-diamino-4-hydroxy-formamido-pyrimidine (Fapy) and 7,8-dihydro-8-oxoguanine (8-oxoG). Although synthetic oligonucleotides containing a single 8-oxoG have been widely used to study enzymatic processing of this lesion, the synthesis of oligonucleotides containing Fapy as a unique lesion has not been achieved to date. In this study, an oligonucleotide containing a single 2,6-diamino-4-hydroxy-5-(N-methyl)formamido-pyrimidine (me-Fapy, a methylated derivative of Fapy) was prepared by a DNA polymerase reaction and the subsequent alkali treatment. The repair activity of Fpg and hOGG1 proteins were compared using oligonucleotide substrates containing me-Fapy and 8-oxoG. |
| Links | |
| Keywords |
DNA Damage; DNA Repair; DNA-Formamidopyrimidine Glycosylase; Escherichia coli/enzymology; Escherichia coli Proteins; Guanine/analogs & derivatives; Guanine/metabolism; Humans; N-Glycosyl Hydrolases/metabolism; Oligodeoxyribonucleotides/chemistry; Oligodeoxyribonucleotides/metabolism; Pyrimidines/metabolism; Species Specificity; Substrate Specificity |
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