PMID:12897023
| Citation |
Calmann, MA and Marinus, MG (2003) Regulated expression of the Escherichia coli dam gene. J. Bacteriol. 185:5012-4 |
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| Abstract |
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease. |
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| Keywords |
ATP-Dependent Proteases; Arabinose/metabolism; Culture Media; DNA Methylation; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Heat-Shock Proteins/metabolism; Mutation; Promoter Regions, Genetic; Protease La; Serine Endopeptidases/metabolism; Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics; Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism; Transcription, Genetic |
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