PMID:12897022
| Citation |
Valenski, ML, Harris, SL, Spears, PA, Horton, JR and Orndorff, PE (2003) The Product of the fimI gene is necessary for Escherichia coli type 1 pilus biosynthesis. J. Bacteriol. 185:5007-11 |
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| Abstract |
Site-directed mutagenesis was employed to create lesions in fimI, a gene of uncertain function located in the chromosomal gene cluster (fim) involved in Escherichia coli type 1 pilus biosynthesis. Chromosomal fimI mutations produced a piliation-negative phenotype. Complementation analysis indicated that a fimI'-kan insertion mutation and a fimI frameshift mutation produced polarity-like effects not seen with an in-frame fimI deletion mutation. Minicell analysis associated fimI with a 16.4-kDa noncytoplasmic protein product (FimI). We conclude that FimI has a required role in normal pilus biosynthesis. |
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| Keywords |
Adhesins, Escherichia coli/genetics; Adhesins, Escherichia coli/metabolism; Alleles; Escherichia coli/genetics; Escherichia coli/metabolism; Fimbriae Proteins/genetics; Fimbriae Proteins/metabolism; Fimbriae, Bacterial/metabolism; Frameshift Mutation; Genetic Complementation Test; Molecular Sequence Data; Mutagenesis, Insertional; Mutagenesis, Site-Directed |
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