PMID:12885413

Citation	Gully, D, Moinier, D, Loiseau, L and Bouveret, E (2003) New partners of acyl carrier protein detected in Escherichia coli by tandem affinity purification. FEBS Lett. 548:90-6
Abstract	We report the first use of tandem affinity purification (TAP) in a prokaryote to purify native protein complexes, and demonstrate its reliability and power. We purified the acyl carrier protein (ACP) of Escherichia coli, a protein involved in a myriad of metabolic pathways. Besides the identification of several known partners of ACP, we rediscovered ACP/MukB and ACP/IscS interactions already detected but previously disregarded as due to contamination. Here, we demonstrate the specificity of these interactions and characterize them. This suggests that ACP is involved in additional previously unsuspected pathways. Furthermore, this study shows how the TAP method can be simply used in prokaryotes such as E. coli to identify new partners in protein-protein interactions under physiological conditions and thereby uncover novel protein functions.
Links	PubMed
Keywords	Acyl Carrier Protein/isolation & purification; Acyl Carrier Protein/metabolism; Affinity Labels; Bacterial Proteins/metabolism; Carbon-Sulfur Lyases/metabolism; Chromosomal Proteins, Non-Histone; Disulfides; Escherichia coli Proteins/isolation & purification; Escherichia coli Proteins/metabolism; Methods; Microspheres; Protein Binding; Serine
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