PMID:12882568
| Citation |
Raghunathan, A and Palsson, BO (2003) Scalable method to determine mutations that occur during adaptive evolution of Escherichia coli. Biotechnol. Lett. 25:435-41 |
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| Abstract |
Denaturing HPLC was used to determine mutations occurring during the adaptive evolution of Escherichia coli K-12. The strains were evolved over 700 generations on glycerol as the sole carbon source from a sub-optimal to an optimal growth rate. The mutations detected by direct sequencing of amplicons of the glycerol-phosphate regulon repressor (glpR) gene were a synonymous substitution Val20Val in two separately evolved strains. Non-synonymous substitutions, Val119Gly and Gly179Trp, were also observed in each of the two strains. This procedure can be scaled to determine genome-scale sequence variations that have occurred during adaptive evolution. |
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| Keywords |
Adaptation, Physiological/genetics; Biological Evolution; Chromatography, High Pressure Liquid/methods; DNA Mutational Analysis/methods; Escherichia coli/genetics; Escherichia coli/physiology; Evolution, Molecular; Gene Expression Profiling/methods; Gene Expression Regulation, Bacterial/physiology; Genome, Bacterial; Pilot Projects; Sequence Alignment/methods; Sequence Analysis, DNA/methods |
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