PMID:12862460

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Citation

Mishina, Y and He, C (2003) Probing the structure and function of the Escherichia coli DNA alkylation repair AlkB protein through chemical cross-linking. J. Am. Chem. Soc. 125:8730-1

Abstract

Engineering chemical cross-linking groups at the protein/DNA interface provide a powerful method for probing the putative active site and a damage searching mechanism of the Escherichia coli alkylation DNA repair protein AlkB.

Links

PubMed Online version:10.1021/ja034636c

Keywords

Alkylation; Base Sequence; Binding Sites; Cross-Linking Reagents/chemistry; DNA Repair; DNA, Bacterial/metabolism; DNA, Single-Stranded/metabolism; Disulfides/chemistry; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Mixed Function Oxygenases/chemistry; Mixed Function Oxygenases/metabolism; Mixed Function Oxygenases/physiology; Structure-Activity Relationship; Zinc/chemistry

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