PMID:12853626
| Citation |
Sauter, C, Basquin, J and Suck, D (2003) Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli. Nucleic Acids Res. 31:4091-8 |
|---|---|
| Abstract |
The Hfq protein was discovered in Escherichia coli in the early seventies as a host factor for the Qbeta phage RNA replication. During the last decade, it was shown to be involved in many RNA processing events and remote sequence homology indicated a link to spliceosomal Sm proteins. We report the crystal structure of the E.coli Hfq protein showing that its monomer displays a characteristic Sm-fold and forms a homo-hexamer, in agreement with former biochemical data. Overall, the structure of the E.coli Hfq ring is similar to the one recently described for Staphylococcus aureus. This confirms that bacteria contain a hexameric Sm-like protein which is likely to be an ancient and less specialized form characterized by a relaxed RNA binding specificity. In addition, we identified an Hfq ortholog in the archaeon Methanococcus jannaschii which lacks a classical Sm/Lsm gene. Finally, a detailed structural comparison shows that the Sm-fold is remarkably well conserved in bacteria, Archaea and Eukarya, and represents a universal and modular building unit for oligomeric RNA binding proteins. |
| Links | |
| Keywords |
Amino Acid Sequence; Autoantigens; Bacteria/genetics; Crystallography, X-Ray; Dimerization; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Evolution, Molecular; Host Factor 1 Protein/chemistry; Host Factor 1 Protein/genetics; Molecular Sequence Data; Protein Conformation; Protein Structure, Secondary; Ribonucleoproteins, Small Nuclear/chemistry; Ribonucleoproteins, Small Nuclear/genetics; Sequence Alignment; Sequence Homology, Amino Acid; snRNP Core Proteins |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.