PMID:12853618
| Citation |
Le Derout, J, Folichon, M, Briani, F, Dehò, G, Régnier, P and Hajnsdorf, E (2003) Hfq affects the length and the frequency of short oligo(A) tails at the 3' end of Escherichia coli rpsO mRNAs. Nucleic Acids Res. 31:4017-23 |
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| Abstract |
Polyadenylation plays an important role in RNA degradation in bacterial cells. In Escherichia coli, exoribonucleases, mostly RNase II and polynucleotide phosphorylase, antagonize the synthesis of poly(A) tails by poly(A) polymerase I (PAP I). In accordance with earlier observations showing that only a small fraction of bacterial RNA is polyadenylated, we demonstrate here that approximately 10% of rpsO mRNA harbors short oligo(A) tails ranging from one to five A residues in wild-type cells. We also compared the length, frequency and distribution of poly(A) tails at the 3'-end of rpsO transcripts in vivo in the presence and absence of Hfq, a host factor that in vitro stimulates the activity of PAP I, and found that Hfq affects all three parameters. In the hfq(+) strain the average length of oligo(A) tails and frequency of polyadenylated transcripts was higher than in the hfq(-) strain and a smaller proportion of tails was found at the 3' end of transcripts terminated at the Rho- independent terminator. Our data led us to the conclusion that Hfq is involved in the recognition of 3' RNA extremities by PAP I. |
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| Keywords |
3' Untranslated Regions/genetics; 3' Untranslated Regions/metabolism; Base Sequence; Endoribonucleases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Exoribonucleases/metabolism; Genotype; Molecular Sequence Data; Nucleic Acid Conformation; Poly A/genetics; Poly A/metabolism; Polyribonucleotide Nucleotidyltransferase/metabolism; RNA, Messenger/chemistry; RNA, Messenger/genetics; RNA, Messenger/metabolism; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Transcription, Genetic |
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