PMID:12829374
| Citation |
Zhang, M, Pradel, N, Mandrand-Berthelot, MA, Yu, Z and Wu, LF (2003) Effect of alteration of the C-terminal extension on the maturation and folding of the large subunit of the Escherichia coli hydrogenase-2. Biochimie 85:575-9 |
|---|---|
| Abstract |
Large subunits of NiFe-hydrogenases undergo a unique maturation process in which the last step consists of the endoproteolytic cleavage of the C-terminal extension after the Ni-Fe metal center has been assembled. To assess in vivo the influence of alteration of the C-terminal extension on the processing, green fluorescence protein (GFP) was fused to the C-terminus of the large subunit (HybC) of the Escherichia coli hydrogenase 2. Interestingly, no processing of HybC-GFP was observed. In addition, the chromophore of GFP was not formed, implying a nonproductive folding of the upstream HybC moiety. These results strongly suggest that the alteration of the C-terminus of the hydrogenase 2 large subunit interferes with the folding and processing of HybC. |
| Links | |
| Keywords |
Amino Acid Sequence; Escherichia coli/enzymology; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/isolation & purification; Escherichia coli Proteins/metabolism; Hydrogenase/chemistry; Hydrogenase/isolation & purification; Hydrogenase/metabolism; Molecular Sequence Data; Nickel/metabolism; Protein Folding; Protein Subunits/chemistry; Protein Subunits/isolation & purification; Protein Subunits/metabolism; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism |
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