PMID:12826280
| Citation |
Connelly, JC, de Leau, ES and Leach, DR (2003) Nucleolytic processing of a protein-bound DNA end by the E. coli SbcCD (MR) complex. DNA Repair (Amst.) 2:795-807 |
|---|---|
| Abstract |
SbcCD and other Mre11/Rad50 (MR) complexes are implicated in the metabolism of DNA ends. They cleave ends sealed by hairpin structures and have been postulated to play roles in removing protein bound to DNA termini. Here we provide direct evidence that the Escherichia coli MR complex (SbcCD) removes protein from a protein-bound DNA end by inserting a double-strand break (DSB). These observations indicate a more complex biochemical action than has been assumed previously and argue that this class of protein has the potential to play a direct role in deprotecting protein-bound DNA ends in vivo. |
| Links | |
| Keywords |
Avidin; Bacterial Proteins/genetics; Biotin; Chromatography, Thin Layer; DNA/metabolism; DNA-Binding Proteins/metabolism; Deoxyribonucleases/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Exonucleases/genetics; Models, Molecular; Oligonucleotides |
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