PMID:12824883
| Citation |
Boulaiz, H, Prados, J, Marchal, JA, García, AM, Alvarez, L, Melguizo, C, Carrillo, E, Ramos, JL and Aránega, A (2003) Transfection of MS-36 melanoma cells with gef gene inhibits proliferation and induces modulation of the cell cycle. Cancer Sci. 94:564-8 |
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| Abstract |
The gef gene, found in Escherichia coli DNA, encodes a small (50 amino acids) protein which is related to cell-killing functions. We used the MS-36 melanoma cell line as an experimental model to examine the usefulness of the gef gene as a new strategy for cancer therapy. We transfected MS-36 cells using the pMAMneo vector, and induced gef gene expression with dexamethasone. This decreased the proliferation rate of MS-36TG by as much as 85% in comparison with MS-36 parental cells. The decrease in cell growth was accompanied with significant modifications of the cell cycle and morphology. The G1-phase gradually disappeared, with accumulation in the S-phase. However, studies with annexin V-FITC and 7-aminoactinomycin D failed to demonstrate induction of apoptosis. Morphological changes were an increase in cell size and the number of filopodia, and especially the appearance of pore-like alterations in the cell membrane which were not seen in parental cells. Our results demonstrate that the gef gene, a system independent of the administration of a prodrug, significantly reduces the proliferation of MS-36 cells. This gene may therefore be considered a new candidate for cancer gene therapy. |
| Links | |
| Keywords |
Apoptosis; Bacterial Toxins/genetics; Bacterial Toxins/metabolism; Cell Division; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; G1 Phase; Genetic Therapy; Humans; Melanoma/metabolism; Melanoma/pathology; Membrane Proteins/genetics; Membrane Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; S Phase; Skin Neoplasms/metabolism; Skin Neoplasms/pathology; Transfection; Tumor Cells, Cultured/metabolism; Tumor Cells, Cultured/ultrastructure |
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