PMID:1279360
| Citation |
Kirchner, J, Lim, D, Witkin, EM, Garvey, N and Roegner-Maniscalco, V (1992) An SOS-inducible defective retronphage (phi R86) in Escherichia coli strain B. Mol. Microbiol. 6:2815-24 |
|---|---|
| Abstract |
In Escherichia coli, RecA protein regulates the DNA damage-inducible survival-enhancing SOS response. Mutant allele recA730, which causes constitutive SOS expression, is lethal at high temperatures in B/r, a derivative of wild-type B, but not in K-12 or in certain B/r--K-12 hybrids. We present evidence that killing is due to SOS induction of a defective retronphage, phi R86, which is integrated into the B/r chromosome at 19 min, but is absent in K-12. phi R86 contains retron EC-86 which encodes reverse transcriptase and a small multicopy DNA-RNA complex, msDNA-RNA. Induction of phi R86 in recA730 B/r strains results in inhibition of host DNA replication before cell death. A retronphage 'killer' gene, ORF336, when overexpressed from a plasmid, causes similar effects without SOS induction. phi R86 is not detectably u.v.-inducible in recA+ strains. |
| Links | |
| Keywords |
Coliphages/genetics; Coliphages/physiology; DNA, Bacterial/biosynthesis; DNA, Single-Stranded/genetics; DNA, Single-Stranded/metabolism; Defective Viruses/genetics; Defective Viruses/physiology; Escherichia coli/genetics; Escherichia coli/physiology; Open Reading Frames; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA-Directed DNA Polymerase/genetics; Rec A Recombinases/metabolism; SOS Response (Genetics); Temperature; Ultraviolet Rays; Viral Plaque Assay; Virus Activation |
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