PMID:12777779
| Citation |
Burman, JD, Stevenson, CE, Hauton, KA, Sawers, G and Lawson, DM (2003) Crystallization and preliminary X-ray analysis of the E. coli hypothetical protein TdcF. Acta Crystallogr. D Biol. Crystallogr. 59:1076-8 |
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| Abstract |
Crystals of the hypothetical protein TdcF (subunit MW = 14 007) from Escherichia coli were grown by vapour diffusion. The protein crystallizes in space group P2(1)2(1)2, with unit-cell parameters a = 72.67, b = 86.22, c = 62.62 A. Native data to a resolution of 2.35 A were collected from a single crystal at 100 K on a rotating-anode X-ray generator. Preliminary analysis of these data indicated that the asymmetric unit corresponded to a trimer, which was supported by a convincing molecular-replacement solution using the YjgF trimer as the probe structure. |
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| Keywords |
Amino Acid Sequence; Amino Acid Transport Systems, Neutral; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Crystallization; Crystallography, X-Ray; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Solvents; X-Ray Diffraction |
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