PMID:12769856
| Citation |
Morimatsu, K and Kowalczykowski, SC (2003) RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair. Mol. Cell 11:1337-47 |
|---|---|
| Abstract |
Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown. Here, we show that the concerted action of the RecFOR complex directs the loading of RecA protein specifically onto gapped DNA that is coated with single-stranded DNA binding (SSB) protein, thereby accelerating DNA strand exchange. The RecFOR complex recognizes the junction between the ssDNA and dsDNA regions and requires a base-paired 5' terminus at the junction. Thus, the RecFOR complex is a structure-specific mediator that targets recombinational repair to ssDNA-dsDNA junctions. This reaction reconstitutes the initial steps of recombinational gapped DNA repair and uncovers an event also common to the repair of ssDNA-tailed intermediates of dsDNA-break repair. We propose that the behavior of the RecFOR proteins is mimicked by functional counterparts that exist in all organisms. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Nucleus/genetics; Cell Nucleus/metabolism; DNA/genetics; DNA/metabolism; DNA Damage/genetics; DNA Repair/genetics; DNA, Single-Stranded/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Eukaryotic Cells/metabolism; Gene Rearrangement/genetics; Nucleoproteins/biosynthesis; Nucleoproteins/genetics; Prokaryotic Cells/metabolism; Rec A Recombinases/genetics; Rec A Recombinases/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.