PMID:12767929
| Citation |
Zhu, Y, Lee, SS and Xu, W (2003) Crystallization and characterization of polyphosphate kinase from Escherichia coli. Biochem. Biophys. Res. Commun. 305:997-1001 |
|---|---|
| Abstract |
Linear polyphosphate chains have been found to play a key role in bacterial responses to stresses and nutritional depletion, and are necessary for host infection of various pathogens. Polyphosphate kinase (PPK) is a critical enzyme responsible for polyphosphate synthesis in bacteria. PPK knockout mutations in several Gram-negative pathogens identify PPK as an ideal drug target for the development of a new class of antibacterial drugs. To reveal the catalytic mechanism and provide a structural basis for drug discovery, we have purified and crystallized full-length Escherichia coli PPK and its complex with AMP-PNP. The crystals diffract to a resolution of 2.5A and belong to the space group P4(2)2(1)2 with unit-cell parameters a=152.0, b=152.0, and c=150.0 A. Crystal structure of PPK is being determined by the Se-Met MAD experiment. |
| Links | |
| Keywords |
Adenylyl Imidodiphosphate/metabolism; Crystallography, X-Ray; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/ultrastructure; Phosphotransferases (Alcohol Group Acceptor)/chemistry; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Phosphotransferases (Alcohol Group Acceptor)/ultrastructure; Phosphotransferases (Phosphate Group Acceptor)/chemistry; Phosphotransferases (Phosphate Group Acceptor)/metabolism; Phosphotransferases (Phosphate Group Acceptor)/ultrastructure; Solubility |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.