PMID:12756233
| Citation |
Ullers, RS, Houben, EN, Raine, A, ten Hagen-Jongman, CM, Ehrenberg, M, Brunner, J, Oudega, B, Harms, N and Luirink, J (2003) Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome. J. Cell Biol. 161:679-84 |
|---|---|
| Abstract |
As newly synthesized polypeptides emerge from the ribosome, they interact with chaperones and targeting factors that assist in folding and targeting to the proper location in the cell. In Escherichia coli, the chaperone trigger factor (TF) binds to nascent polypeptides early in biosynthesis facilitated by its affinity for the ribosomal proteins L23 and L29 that are situated around the nascent chain exit site on the ribosome. The targeting factor signal recognition particle (SRP) interacts specifically with the signal anchor (SA) sequence in nascent inner membrane proteins (IMPs). Here, we have used photocross-linking to map interactions of the SA sequence in a short, in vitro-synthesized, nascent IMP. Both TF and SRP were found to interact with the SA with partially overlapping binding specificity. In addition, extensive contacts with L23 and L29 were detected. Both purified TF and SRP could be cross-linked to L23 on nontranslating ribosomes with a competitive advantage for SRP. The results suggest a role for L23 in the targeting of IMPs as an attachment site for TF and SRP that is close to the emerging nascent chain. |
| Links |
PubMed PMC2199365 Online version:10.1083/jcb.200302130 |
| Keywords |
Bacterial Proteins/metabolism; Escherichia coli/cytology; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Membrane Proteins/metabolism; Peptidylprolyl Isomerase/metabolism; Protein Binding; Protein Biosynthesis; Ribosomal Proteins/metabolism; Ribosomes/metabolism; Signal Recognition Particle/metabolism |
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