PMID:12730187
| Citation |
De Bie, LG, Roovers, M, Oudjama, Y, Wattiez, R, Tricot, C, Stalon, V, Droogmans, L and Bujnicki, JM (2003) The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase. J. Bacteriol. 185:3238-43 |
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| Abstract |
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N(7)-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cell Division/genetics; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Guanosine/analogs & derivatives; Guanosine/metabolism; Molecular Sequence Data; Multigene Family; RNA, Transfer/chemistry; RNA, Transfer/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; S-Adenosylmethionine/metabolism; Sequence Analysis; Sequence Homology, Amino Acid; tRNA Methyltransferases/genetics; tRNA Methyltransferases/metabolism |
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