PMID:12725933
| Citation |
Cheng, B, Rui, S, Ji, C, Gong, VW, Van Dyk, TK, Drolet, M and Tse-Dinh, YC (2003) RNase H overproduction allows the expression of stress-induced genes in the absence of topoisomerase I. FEMS Microbiol. Lett. 221:237-42 |
|---|---|
| Abstract |
Induction of stress proteins in response to heat shock was found to be reduced significantly in Escherichia coli with DeltatopA mutation. RNase H overexpression in the DeltatopA mutant partially restored the sigma(32)-dependent induction of stress genes in response to high temperature and ethanol. The presence of overexpressed RNase H also improved the survival rate of the DeltatopA mutant after high temperature and oxidative challenges. Topoisomerase I is likely required during stress response for preventing accumulation of transcription-driven hypernegative supercoiling and R-loop formation at induced stress genes loci. |
| Links | |
| Keywords |
DNA Topoisomerases, Type I/genetics; DNA, Bacterial/analysis; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Expression Regulation, Bacterial; Heat-Shock Proteins/biosynthesis; Mutation; Nucleic Acid Conformation; Ribonuclease H/biosynthesis; Temperature; Transcription, Genetic |
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