PMID:12722631

Citation	Ovcharova, IV, Eremina, SIu and Mironov, AS (2003) [Determination of functional role of nucleotide composition in the transcription start region of the Escherichia coli udp gene]. Genetika 39:326-35
Abstract	The promoter of the Escherichia coli udp gene contains the poly-T (5'-TTTTT-3') motif in the transcription start region located at the distance of 3 nucleotides with respect to the Pribnow box. By means of site-directed mutagenesis, mutations in the +1, -1, and +3 positions of this region were isolated and their functional role in transcription initiation was determined. It was shown that in addition to the thymine nucleotide earlier identified at position 4 (with respect to the 5' end of the poly-T motif), the third thymine nucleotide may serve as an efficient transcription site. The functional significance of the presence of the poly-T motif in the transcription initiation region is discussed. Analysis of the isolated mutant promoters revealed the ability of the guanine nucleotide at the +3 position to stabilize the formation of a productive transcription complex and to serve as an additional transcription start.
Links	PubMed
Keywords	Base Composition; Base Sequence; Escherichia coli Proteins/genetics; Guanine; Molecular Sequence Data; Mutation; Poly T; Promoter Regions, Genetic; Sequence Homology, Nucleic Acid; Transcription Initiation Site; beta-Galactosidase/genetics
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