PMID:12707259
| Citation |
Chen, M, Xie, K, Nouwen, N, Driessen, AJ and Dalbey, RE (2003) Conditional lethal mutations separate the M13 procoat and Pf3 coat functions of YidC: different YIDC structural requirements for membrane protein insertion. J. Biol. Chem. 278:23295-300 |
|---|---|
| Abstract |
Conditional lethal YidC mutants have been isolated to decipher the role of YidC in the assembly of Sec-dependent and Sec-independent membrane proteins. We now show that the membrane insertion of the Sec-independent M13 procoat-lep protein is inhibited in a short time in a temperature-sensitive mutant when shifted to the nonpermissive temperature. This provides an additional line of evidence that YidC plays a direct role in the insertion of the Sec-independent M13 procoat protein. However, in the temperature-sensitive mutant, the insertion of the Sec-independent Pf3 phage coat protein and the Sec-dependent leader peptidase were not strongly inhibited at the restricted temperatures. Conversely, using a cold-sensitive YidC strain, we find that the membrane insertion of the Sec-independent Pf3 coat protein is blocked, and the Sec-dependent leader peptidase is inhibited at the nonpermissive temperature, whereas the insertion of the M13 procoat protein is nearly normal. These data show that the YidC function for procoat and its function for Pf3 coat and possibly leader peptidase are genetically separable and suggest that the YidC structural requirements are different for the Sec-independent M13 procoat and Pf3 coat phage proteins that insert by different mechanisms. |
| Links |
PubMed Online version:10.1074/jbc.M301008200 |
| Keywords |
Adenosine Triphosphatases/physiology; Bacterial Proteins; Bacteriophage M13/chemistry; Bacteriophage M13/physiology; Capsid/metabolism; Capsid/physiology; Capsid Proteins; Escherichia coli/virology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/physiology; Genes, Lethal/physiology; Membrane Proteins; Membrane Transport Proteins/genetics; Membrane Transport Proteins/physiology; Mutation; Protein Precursors/metabolism; Protein Precursors/physiology; Protein Sorting Signals; Protein Transport; Serine Endopeptidases; Temperature |
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