PMID:12702451
| Citation |
Richard, P, Verho, R, Putkonen, M, Londesborough, J and Penttilä, M (2003) Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway. FEMS Yeast Res. 3:185-9 |
|---|---|
| Abstract |
The fungal pathway for L-arabinose catabolism converts L-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed. |
| Links | |
| Keywords |
Anaerobiosis; Arabinose/metabolism; Ethanol/metabolism; Fermentation/physiology; Oxidation-Reduction; Pentosephosphates/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism |
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