PMID:12699743
| Citation |
Chen, LS, Bahr, MH and Sheppard, TL (2003) Effects of 8-chlorodeoxyadenosine on DNA synthesis by the Klenow fragment of DNA polymerase I. Bioorg. Med. Chem. Lett. 13:1509-12 |
|---|---|
| Abstract |
8-chloro-2'-deoxyadenosine (8-Cl-dAdo) was incorporated into synthetic DNA oligonucleotides to determine its effects on DNA synthesis by the 3'-5' exonuclease-free Klenow fragment of Escherichia coli DNA Polymerase I (KF-). Single nucleotide insertion experiments were used to determine the coding potential of 8-Cl-dAdo in a DNA template. KF- inserted TTP opposite 8-Cl-dAdo in the template, but with decreased efficiency relative to natural deoxyadenosine. Running-start primer extensions with KF- resulted in polymerase pausing at 8-Cl-dAdo template sites during DNA synthesis. The 2'-deoxyribonucleoside triphosphate analogue, 8-Cl-dATP, was incorporated opposite thymidine (T) approximately two-fold less efficiently than dATP. |
| Links | |
| Keywords |
DNA/chemical synthesis; DNA Polymerase I/chemistry; Deoxyadenosines/pharmacology; Escherichia coli/chemistry; Oligonucleotides/chemistry; Thymidine/chemistry |
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