PMID:12682041
| Citation |
Lillig, CH, Potamitou, A, Schwenn, JD, Vlamis-Gardikas, A and Holmgren, A (2003) Redox regulation of 3'-phosphoadenylylsulfate reductase from Escherichia coli by glutathione and glutaredoxins. J. Biol. Chem. 278:22325-30 |
|---|---|
| Abstract |
Inorganic sulfate (SO42-, S+VI) is reduced in vivo to sulfite (SO32-, S+IV) via phosphoadenylylsulfate (PAPS) reductase. Escherichia coli lacking glutathione reductase and glutaredoxins (gor-grxA-grxB-grxC-) barely grows on sulfate. We found that incubation of PAPS reductase with oxidized glutathione leads to enzyme inactivation with simultaneous formation of a mixed disulfide between glutathione and the active site Cys-239. A newly developed method based on thiol-specific fluorescent alkylation and gel electrophoresis showed that glutathionylated PAPS reductase is reduced by glutaredoxins via a monothiol mechanism. This glutathionylated species was also observed in poorly growing gor-grxA-grxB-grxC- cells expressing inactive glutaredoxin 2 (Grx2) C9S/C12S. However, it was absent in better growing cells expressing monothiol Grx2 C12S or wild type Grx2. Reversible glutathionylation may thus regulate the activity of PAPS reductase in vivo. |
| Links |
PubMed Online version:10.1074/jbc.M302304200 |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Deletion; Glutaredoxins; Glutathione/pharmacology; Glutathione Disulfide/pharmacology; Kinetics; Oxidoreductases/antagonists & inhibitors; Oxidoreductases/chemistry; Oxidoreductases/metabolism; Peptide Fragments/chemistry; Proteins/genetics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.