PMID:12673622
| Citation |
Dudás, A, Marková, E, Vlasáková, D, Kolman, A, Bartosová, Z, Brozmanová, J and Chovanec, M (2003) The Escherichia coli RecA protein complements recombination defective phenotype of the Saccharomyces cerevisiae rad52 mutant cells. Yeast 20:389-96 |
|---|---|
| Abstract |
The Saccharomyces cerevisiae rad52 mutants are sensitive to many DNA damaging agents, mainly to those that induce DNA double-strand breaks (DSBs). In the yeast, DSBs are repaired primarily by homologous recombination (HR). Since almost all HR events are significantly reduced in the rad52 mutant cells, the Rad52 protein is believed to be a key component of HR in S. cerevisiae. Similarly to the S. cerevisiae Rad52 protein, RecA is the main HR protein in Escherichia coli. To address the question of whether the E. coli RecA protein can rescue HR defective phenotype of the rad52 mutants of S. cerevisiae, the recA gene was introduced into the wild-type and rad52 mutant cells. Cell survival and DSBs induction and repair were studied in the RecA-expressing wild-type and rad52 mutant cells after exposure to ionizing radiation (IR) and methyl methanesulphonate (MMS). Here, we show that expression of the E. coli RecA protein partially complemented sensitivity and fully complemented DSB repair defect of the rad52 mutant cells after exposure to IR and MMS. We suggest that in the absence of Rad52, when all endogenous HR mechanisms are knocked out in S. cerevisiae, the heterologous E. coli RecA protein itself presumably takes over the broken DNA. |
| Links |
PubMed Online version:10.1002/yea.971 |
| Keywords |
Chromosomes, Fungal/metabolism; DNA Damage/genetics; DNA Repair/genetics; DNA Repair/physiology; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Electrophoresis, Gel, Pulsed-Field; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Genetic Complementation Test; Mutagenesis, Insertional; Rad52 DNA Repair and Recombination Protein; Rec A Recombinases/biosynthesis; Rec A Recombinases/genetics; Recombination, Genetic/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism |
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