PMID:12655407
| Citation |
Matsuo, E, Mori, H and Ito, K (2003) Interfering mutations provide in vivo evidence that Escherichia coli SecE functions in multimeric states. Mol. Genet. Genomics 268:808-15 |
|---|---|
| Abstract |
SecY, SecE and SecG form a heterotrimer, which functions as a protein translocation channel in Escherichia coli. The cytosolic loop of SecE contains a segment that is conserved among different organisms. Here we show that mutational alterations in this segment not only inactivate the SecE function but confer dominant interfering properties on the altered SecE molecule. Such effects were especially evident in mutant cells in which the requirement for SecE function was increased. Overproduction of SecE, but not of SecY, alleviated the dominant negative effects. These results suggest that the inactive SecE molecule sequesters wild-type SecE. It was also found that an amino acid substitution, D112P, in the C-terminal periplasmic region intragenically suppressed the dominant interference. These results are consistent with a notion that there is significant SecE-SecE interaction in vivo, in which the C-terminal region has an important role. The data hence suggest that dimeric SecE participates in the formation of the functional translocation channel. |
| Links |
PubMed Online version:10.1007/s00438-003-0803-9 |
| Keywords |
Amino Acid Sequence; Amino Acid Substitution; Base Sequence; DNA, Bacterial/genetics; Dimerization; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Plasmids/genetics; Protein Transport; Sequence Deletion |
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