PMID:12653962
| Citation |
Matsumura, T, Yuasa, T, Hayashi, T, Obara, T, Kimata, Y and Yanagida, M (2003) A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids. Genes Cells 8:341-55 |
|---|---|
| Abstract |
The fission yeast Schizosaccharomyces pombe separase/Cut1 and securin/Cut2 are required for anaphase-specific activation of proteolysis that leads to proper sister chromatid separation. We intended to identify ts (temperature sensitive) strains whose growth was inhibited by multicopy plasmid pCUT1 or pCUT2 at the permissive temperature. |
| Links | |
| Keywords |
Cell Cycle Proteins/biosynthesis; Cloning, Molecular; Endopeptidases/biosynthesis; Enzyme Inhibitors; Mitosis; Mutation; Plasmids/metabolism; Schizosaccharomyces/cytology; Schizosaccharomyces/enzymology; Schizosaccharomyces/genetics; Schizosaccharomyces/metabolism; Schizosaccharomyces pombe Proteins/biosynthesis; Schizosaccharomyces pombe Proteins/genetics; Temperature; Ubiquitin-Protein Ligase Complexes/antagonists & inhibitors; Ubiquitin-Protein Ligase Complexes/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.