PMID:12644833
| Citation |
Deckert, PM, Renner, C, Cohen, LS, Jungbluth, A, Ritter, G, Bertino, JR, Old, LJ and Welt, S (2003) A33scFv-cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. Br. J. Cancer 88:937-9 |
|---|---|
| Abstract |
A recombinant fusion protein of colon carcinoma binding A33 single chain antibody with cytosine deaminase displayed specific antigen binding and enzyme activity in surface plasmon resonance and is catalytic activity assay. In vitro, it selectively increased the toxicity of 5-FC to A33 antigen-positive cells by 300-fold, demonstrating the potency of this ADEPT strategy. |
| Links |
PubMed PMC2377093 Online version:10.1038/sj.bjc.6600751 |
| Keywords |
Antigens, Neoplasm; Catalysis; Cytosine Deaminase; Escherichia coli/genetics; Humans; Immunoglobulin Fragments; Immunoglobulin Variable Region; Membrane Glycoproteins/immunology; Membrane Glycoproteins/pharmacology; Nucleoside Deaminases/chemistry; Nucleoside Deaminases/immunology; Nucleoside Deaminases/pharmacology; Polymerase Chain Reaction; Recombinant Fusion Proteins/pharmacology; Tumor Cells, Cultured |
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