PMID:12644513
| Citation |
Enz, S, Mahren, S, Menzel, C and Braun, V (2003) Analysis of the ferric citrate transport gene promoter of Escherichia coli. J. Bacteriol. 185:2387-91 |
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| Abstract |
FecI, an extracytoplasmic-function sigma factor, is required for initiation of transcription of the ferric citrate transport genes. A mutational analysis of the fecA promoter revealed that the nonconserved -10 region and a downstream regulatory element are important for fecA promoter activity. However, nucleotide substitutions in the well-conserved -35 region also have an effect on the fecA promoter activity. Titration of FecI suggests that the FecI-RNA polymerase holoenzyme does not bind strongly to the downstream regulatory element, which is therefore probably involved in a subsequent step of transcription initiation. |
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| Keywords |
Base Sequence; Biological Transport; Carrier Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Ferric Compounds/metabolism; Green Fluorescent Proteins; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Molecular Sequence Data; Point Mutation; Promoter Regions, Genetic; Receptors, Cell Surface; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Transcription Initiation Site |
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