PMID:12624954
| Citation |
Soboleva, NG, Makhno, VI, Konevega, AL, Semenkov, IuP and Katunin, VI [The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the A-site of the 70S ribosome]. Mol. Biol. (Mosk.) 37:121-7 |
|---|---|
| Abstract |
To estimate the effect of modified nucleotide-37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNAK+YPhe and Phe-tRNAK-YPhe) with the A site of complex [70S.poly(U).deacylated tRNA(Phe) in the P site] was assayed at 0-20 degrees C. As comparisons with native Phe-tRNAK+YPhe showed, removal of the Y base decreased the association constant of Phe-tRNAK-YPhe and the complex by an order of magnitude at any temperature, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNA(Phe) bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNAK-YPhe but not for Phe-tRNAK+YPhe. Thus, the modified nucleotide 3' of the Phe-tRNA(Phe) anticodon stabilized the codon-anticodon interaction both in the A and in the P sites of the 70S ribosome. |
| Links | |
| Keywords |
Anticodon; Binding Sites; Escherichia coli/genetics; Mutagenesis, Site-Directed; Nucleotides/genetics; RNA, Transfer, Amino Acyl/genetics; RNA, Transfer, Amino Acyl/metabolism; RNA, Transfer, Phe/genetics; RNA, Transfer, Phe/metabolism; Ribosomes/genetics; Ribosomes/metabolism |
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