PMID:12596879
| Citation |
Fujita, C, Maeda, M, Fujii, T, Iwamoto, R and Ikehara, K (2002) Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein. Biosci. Biotechnol. Biochem. 66:2735-8 |
|---|---|
| Abstract |
Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293rd position did not restore cell growth of E. coli CF8295 (delta relA, delta spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein. |
| Links | |
| Keywords |
Alanine/genetics; Amino Acid Sequence; Amino Acid Substitution; Aspartic Acid/genetics; Cell Division; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Guanosine Tetraphosphate/biosynthesis; Guanosine Tetraphosphate/metabolism; Molecular Sequence Data; Mutation; Pyrophosphatases/chemistry; Pyrophosphatases/genetics; Pyrophosphatases/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.