PMID:12595738
| Citation |
Horsefield, R, Yankovskaya, V, Törnroth, S, Luna-Chavez, C, Stambouli, E, Barber, J, Byrne, B, Cecchini, G and Iwata, S (2003) Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 59:600-2 |
|---|---|
| Abstract |
The membrane-bound respiratory complex II, succinate:ubiquinone oxidoreductase (SQR) from Escherichia coli, has been anaerobically expressed, then purified and crystallized. The initial crystals obtained were small and diffracted poorly. In order to facilitate structure determination, rational screening and sample-quality analysis using electron microscopy was implemented. The crystals of SQR from E. coli belong to the trigonal space group R32, with unit-cell parameters a = b = 138.7, c = 521.9 A, and diffract to 2.6 A resolution. The optimization strategy used for obtaining well diffracting SQR crystals is applicable to a wide range of membrane proteins. |
| Links | |
| Keywords |
Anisotropy; Coloring Agents; Crystallization; Crystallography, X-Ray; Electron Transport Complex II; Escherichia coli/enzymology; Microscopy, Electron; Multienzyme Complexes/chemistry; Multienzyme Complexes/isolation & purification; Oxidoreductases/chemistry; Oxidoreductases/isolation & purification; Succinate Dehydrogenase/chemistry; Succinate Dehydrogenase/isolation & purification; Ultracentrifugation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.