PMID:12595710

Citation	Chimento, DP, Mohanty, AK, Kadner, RJ and Wiener, MC (2003) Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 59:509-11
Abstract	BtuB, the cobalamin transporter from Escherichia coli, has been overexpressed, purified and crystallized. The purified protein was solubilized in n-octyl tetraoxyethylene (C(8)E(4)) and was crystallized using sitting-drop vapor diffusion with PEG 3350 and magnesium acetate as precipitants (pH 6.5). Two crystal forms have been obtained. Crystal type I belongs to space group P3(1)21, with unit-cell parameters a = b = 81.6, c = 210.0 A, alpha = beta = 90, gamma = 120 degrees. Crystal type II belongs to space group P3(1)21, with unit-cell parameters a = b = 81.6, c = 226.0 A, alpha = beta = 90, gamma = 120 degrees. Each crystal form contains a monomer in the asymmetric unit. Diffraction for crystal type I extends to 2.0 A and diffraction for crystal type II extends to 2.7 A. Both crystal forms are suitable for structure determination.
Links	PubMed
Keywords	Bacterial Outer Membrane Proteins; Cloning, Molecular; Crystallization; Electrophoresis, Polyacrylamide Gel; Escherichia coli/chemistry; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/chemistry; Membrane Transport Proteins; Receptors, Peptide/biosynthesis; Receptors, Peptide/chemistry; Vitamin B 12/metabolism; X-Ray Diffraction
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