PMID:12578351

Citation	Fanucci, GE, Coggshall, KA, Cadieux, N, Kim, M, Kadner, RJ and Cafiso, DS (2003) Substrate-induced conformational changes of the periplasmic N-terminus of an outer-membrane transporter by site-directed spin labeling. Biochemistry 42:1391-400
Abstract	The structure and dynamics of the N-terminal and core regions of BtuB, an outer membrane vitamin B(12) transporter from Escherichia coli, were investigated by site-directed spin labeling. Cysteine mutants were generated by site-directed mutagenesis to place spin labels in the N-terminal region (residues 1-17), the core region (residues 25-30), and double labels into the Ton box (residues 6-12). BtuB mutants were expressed, spin labeled, purified, and reconstituted into phosphatidylcholine. In the presence of substrate (vitamin B(12)), EPR spectroscopy demonstrates that there is a conformational change in the Ton box similar to that seen previously for BtuB in intact outer membranes. The Ton box is positioned within the beta-barrel of BtuB in the absence of substrate (docked configuration) but becomes unfolded and increases its aqueous exposure upon substrate binding (undocked configuration). This conformational change and the similarity in the EPR spectra between reconstituted and native membranes indicate that BtuB is correctly folded and functional in the reconstituted system. The protein segment on the N-terminal side of the Ton box is highly mobile, and it becomes more mobile in the presence of substrate. Side chains in the region C-terminal to the Ton box also show increases in mobility with substrate addition, but position 16 appears to define a hinge point for this conformation change. EPR line shapes and relaxation data indicate that residues 25-30 form a beta-strand structure, which is analogous to the first beta-strand in the cores of the homologous iron transporters. When substrate binds to BtuB, this first beta-strand remains folded. The EPR spectra of double-nitroxide labels within the Ton box are broadened because of dipolar and collisional exchange interactions. The broadening pattern indicates that the Ton box is not helical but is in an extended or beta-strand structure.
Links	PubMed Online version:10.1021/bi027120z
Keywords	Bacterial Outer Membrane Proteins/chemistry; Bacterial Outer Membrane Proteins/genetics; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Biological Transport/genetics; Electron Spin Resonance Spectroscopy/methods; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Transport Proteins; Mutagenesis, Site-Directed; Peptide Fragments/chemistry; Peptide Fragments/genetics; Periplasmic Proteins/chemistry; Periplasmic Proteins/genetics; Protein Conformation; Protein Structure, Secondary/genetics; Receptors, Peptide/chemistry; Receptors, Peptide/genetics; Spin Labels; Substrate Specificity/genetics; Thermodynamics; Vitamin B 12/chemistry
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