PMID:12575933

Citation	Heras, B, Edeling, MA, Byriel, KA, Jones, A, Raina, S and Martin, JL (2003) Dehydration converts DsbG crystal diffraction from low to high resolution. Structure 11:139-45
Abstract	Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 A resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 A resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.
Links	PubMed
Keywords	Crystallography, X-Ray; Desiccation; Escherichia coli Proteins/chemistry; Glycerol/metabolism; Oxidoreductases/chemistry; Periplasmic Proteins/chemistry; Water/chemistry
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