PMID:12568936

Citation	Powell, LM, Lejeune, E, Hussain, FS, Cronshaw, AD, Kelly, SM, Price, NC and Dryden, DT (2003) Assembly of EcoKI DNA methyltransferase requires the C-terminal region of the HsdM modification subunit. Biophys. Chem. 103:129-37
Abstract	The methyltransferase component of type I DNA restriction and modification systems comprises three subunits, one DNA sequence specificity subunit and two DNA modification subunits. Limited proteolysis of the EcoKI methyltransferase shows that a 55-kDa N-terminal fragment of the 59-kDa modification subunit is resistant to degradation. We have purified this fragment and determined by mass spectrometry that proteolysis removes 43 or 44 amino acids from the C-terminus. The fragment fails to interact with the other subunits even though it still possesses secondary and tertiary structure and the ability to bind the S-adenosylmethionine cofactor. We conclude that the C-terminal region of the modification subunit of EcoKI is essential for the assembly of the EcoKI methyltransferase.
Links	PubMed
Keywords	Amino Acid Sequence; Peptide Fragments/chemistry; Peptide Fragments/isolation & purification; Peptide Fragments/metabolism; Peptide Mapping; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Subunits/chemistry; Protein Subunits/metabolism; Site-Specific DNA-Methyltransferase (Adenine-Specific)/biosynthesis; Site-Specific DNA-Methyltransferase (Adenine-Specific)/chemistry
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