PMID:12562818
| Citation |
Matic, I, Babic, A and Radman, M (2003) 2-aminopurine allows interspecies recombination by a reversible inactivation of the Escherichia coli mismatch repair system. J. Bacteriol. 185:1459-61 |
|---|---|
| Abstract |
2-Aminopurine treatment of Escherichia coli induces a reversible phenotype of DNA mismatch repair deficiency. This transient phenotype results in a 300-fold increase in the frequency of interspecies conjugational recombination with a Salmonella enterica serovar Typhimurium Hfr donor. This method can be used for the generation of biodiversity by allowing recombination between diverged genes and genomes. |
| Links | |
| Keywords |
2-Aminopurine/pharmacology; Adenosine Triphosphatases/genetics; Bacterial Proteins; Base Pair Mismatch; Conjugation, Genetic/drug effects; DNA Repair; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli Proteins/genetics; MutS DNA Mismatch-Binding Protein; Recombination, Genetic/drug effects; Salmonella typhimurium/genetics |
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