PMID:12543983
| Citation |
Courcelle, J, Donaldson, JR, Chow, KH and Courcelle, CT (2003) DNA damage-induced replication fork regression and processing in Escherichia coli. Science 299:1064-7 |
|---|---|
| Abstract |
DNA lesions that block replication are a primary cause of rearrangements, mutations, and lethality in all cells. After ultraviolet (UV)-induced DNA damage in Escherichia coli, replication recovery requires RecA and several other recF pathway proteins. To characterize the mechanism by which lesion-blocked replication forks recover, we used two-dimensional agarose gel electrophoresis to show that replication-blocking DNA lesions induce a transient reversal of the replication fork in vivo. The reversed replication fork intermediate is stabilized by RecA and RecF and is degraded by the RecQ-RecJ helicase-nuclease when these proteins are absent. We propose that fork regression allows repair enzymes to gain access to the replication-blocking lesion, allowing processive replication to resume once the blocking lesion is removed. |
| Links |
PubMed Online version:10.1126/science.1081328 |
| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA Damage; DNA Helicases/metabolism; DNA Repair; DNA Replication; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Electrophoresis, Agar Gel; Electrophoresis, Gel, Two-Dimensional; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/radiation effects; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Exodeoxyribonucleases/metabolism; Nucleic Acid Conformation; Plasmids/metabolism; Rec A Recombinases/genetics; Rec A Recombinases/metabolism; RecQ Helicases; Ultraviolet Rays |
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