PMID:12533486
| Citation |
Reyes-Domínguez, Y, Contreras-Ferrat, G, Ramírez-Santos, J, Membrillo-Hernández, J and Gómez-Eichelmann, MC (2003) Plasmid DNA supercoiling and gyrase activity in Escherichia coli wild-type and rpoS stationary-phase cells. J. Bacteriol. 185:1097-100 |
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| Abstract |
Stationary-phase cells displayed a distribution of relaxed plasmids and had the ability to recover plasmid supercoiling as soon as nutrients became available. Preexisting gyrase molecules in these cells were responsible for this recovery. Stationary-phase rpoS cells showed a bimodal distribution of plasmids and failed to supercoil plasmids after the addition of nutrients, suggesting that rpoS plays a role in the regulation of plasmid topology during the stationary phase. |
| Links | |
| Keywords |
Bacterial Proteins/physiology; DNA Gyrase/metabolism; DNA, Superhelical/chemistry; Escherichia coli/genetics; Hydrogen-Ion Concentration; Ligases/physiology; Plasmids/genetics; Sigma Factor/physiology; Transcription, Genetic |
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