PMID:12527378
| Citation |
Ray, N, Oates, J, Turner, RJ and Robinson, C (2003) DmsD is required for the biogenesis of DMSO reductase in Escherichia coli but not for the interaction of the DmsA signal peptide with the Tat apparatus. FEBS Lett. 534:156-60 |
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| Abstract |
The DmsD protein is essential for the biogenesis of DMSO reductase in Escherichia coli, and binds the signal peptide of the DmsA subunit, a Tat substrate. This suggests a role as a guidance factor to target pre-DmsA to the translocase. Here, we have analysed the export of fusion proteins in which the DmsA and TorA signal peptides are fused to green fluorescent protein. Both chimeras are efficiently exported to the periplasm in wild-type E. coli cells and we show that their export efficiencies are essentially identical in a mutant lacking DmsD. An authentic Tat substrate, TMAO reductase, is also efficiently exported in the dmsD mutant. The data indicate that DmsD carries out a critical role in DMSO reductase biogenesis/assembly but is not required for the functioning of the DmsA signal peptide. |
| Links | |
| Keywords |
Carrier Proteins/genetics; Carrier Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Green Fluorescent Proteins; Iron-Sulfur Proteins; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutation; Oxidoreductases/genetics; Oxidoreductases/metabolism; Oxidoreductases, N-Demethylating/genetics; Oxidoreductases, N-Demethylating/metabolism; Periplasm/metabolism; Protein Sorting Signals; Protein Transport; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
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