PMID:12514740
| Citation |
Lee, YH, Nadaraia, S, Gu, D, Becker, DF and Tanner, JJ (2003) Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein. Nat. Struct. Biol. 10:109-14 |
|---|---|
| Abstract |
The PutA flavoprotein from Escherichia coli plays multiple roles in proline catabolism by functioning as a membrane-associated bi-functional enzyme and a transcriptional repressor of proline utilization genes. The human homolog of the PutA proline dehydrogenase (PRODH) domain is critical in p53-mediated apoptosis and schizophrenia. Here we report the crystal structure of a 669-residue truncated form of PutA that shows both PRODH and DNA-binding activities, representing the first structure of a PutA protein and a PRODH enzyme from any organism. The structure is a domain-swapped dimer with each subunit comprising three domains: a helical dimerization arm, a 120-residue domain containing a three-helix bundle similar to that in the helix-turn-helix superfamily of DNA-binding proteins and a beta/alpha-barrel PRODH domain with a bound lactate inhibitor. Analysis of the structure provides insight into the mechanism of proline oxidation to pyrroline-5-carboxylate, and functional studies of a mutant protein suggest that the DNA-binding domain is located within the N-terminal 261 residues of E. coli PutA. |
| Links |
PubMed PMC3727246 Online version:10.1038/nsb885 |
| Keywords |
Bacterial Proteins/chemistry; Catalytic Domain; Crystallography, X-Ray; DNA-Binding Proteins/chemistry; Dimerization; Escherichia coli Proteins/chemistry; Humans; Membrane Proteins/chemistry; Models, Molecular; Proline Oxidase/chemistry; Protein Conformation; Protein Structure, Quaternary; Protein Structure, Tertiary |
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