PMID:12422512
| Citation |
Karpiński, P, Grudniak, A and Wolska, KI (2002) Effect of mutations in dnaK and dnaJ genes on cysteine operon expression in Escherichia coli. Folia Microbiol. (Praha) 47:371-4 |
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| Abstract |
The effect of mutations in dnaK and dnaJ genes on the expression of two operons that are part of cysteine regulon was determined using Escherichia coli strains harboring cysPTWA::lacZ and cysJIH::lacZ fusions. Null dnaJ and dnaKdnaJ mutants were impaired in beta-galactosidase expression from both fusions. Efficient complementation of this defect by wild-type alleles present on a low-copy number plasmid was achieved. The presence of the pMH224 plasmid coding for CysB* protein defective in DNA binding lowered beta-galactosidase expression from cysPTWA::lacZ fusion strain harboring wild-type dnaKdnaJ alleles but did not diminish enzyme expression in delta dnaJ and delta dnaKdnaJ strains. |
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| Keywords |
Alleles; Artificial Gene Fusion; Cysteine/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Genetic Complementation Test; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins/genetics; Heat-Shock Proteins/genetics; Lac Operon; Mutation; Operon; Plasmids/genetics; beta-Galactosidase/genetics |
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