PMID:12421318
| Citation |
Weigel, C and Seitz, H (2002) Strand-specific loading of DnaB helicase by DnaA to a substrate mimicking unwound oriC. Mol. Microbiol. 46:1149-56 |
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| Abstract |
We analysed the enzymatic activity (strand dis-placement) of the Escherichia coli DnaB helicase on a mirror-image pair of oligonucleotide-based substrates mimicking the unwound replication origin oriC. Loading of the helicase complex occurred exclusively to the single-stranded 'lower strand' part of the substrates. Full helicase activity required DnaA bound to the double-stranded part of the substrates (oriC DnaA box R1) and to their single-stranded 'upper strand' part. We assume that in vivo DnaA also loads the first of two helicase complexes - required for the assembly of two replication forks - to the lower strand of oriC during initiation of bidirectional chromosome replication in E. coli. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Base Sequence; DNA/chemistry; DNA/metabolism; DNA Helicases/metabolism; DNA Replication; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; DnaB Helicases; Escherichia coli/genetics; Models, Chemical; Models, Molecular; Molecular Mimicry; Molecular Sequence Data; Oligonucleotides/chemistry; Oligonucleotides/metabolism; Origin Recognition Complex; Viral Proteins/chemistry; Viral Proteins/metabolism |
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