PMID:12408833
| Citation |
Yamada, K, Miyata, T, Tsuchiya, D, Oyama, T, Fujiwara, Y, Ohnishi, T, Iwasaki, H, Shinagawa, H, Ariyoshi, M, Mayanagi, K and Morikawa, K (2002) Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery. Mol. Cell 10:671-81 |
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| Abstract |
We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode. |
| Links | |
| Keywords |
Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/metabolism; Adenosine Triphosphatases/ultrastructure; Adenylyl Imidodiphosphate/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Bacterial Proteins/ultrastructure; Crystallography, X-Ray; DNA Helicases/chemistry; DNA Helicases/metabolism; DNA Helicases/ultrastructure; DNA Repair; DNA Replication; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/ultrastructure; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/ultrastructure; Models, Molecular; Molecular Motor Proteins/chemistry; Molecular Motor Proteins/metabolism; Molecular Motor Proteins/ultrastructure; Multienzyme Complexes; Nucleic Acid Conformation; Protein Binding; Protein Structure, Tertiary; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Recombination, Genetic |
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