PMID:12402320
| Citation |
Gerigk, M, Bujnicki, R, Ganpo-Nkwenkwa, E, Bongaerts, J, Sprenger, G and Takors, R (2002) Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains. Biotechnol. Bioeng. 80:746-54 |
|---|---|
| Abstract |
A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback resistant DAHP-synthase (3-desoxy-D-arabino-heptusonate-7-phosphate)) or the wild-type aroF(wt) were used as model systems to elucidate the necessity of finding an individual process optimum for each genotype. With the aid of tyrosine control, wild-type aroF(wt) could be used for L-Phe production achieving higher final L-Phe titers (34 g/L) than the aroF(fbr) strain (28 g/L) and providing higher DAHP-synthase activities. With on-line glucose control, an optimum glucose concentration of 5 g/L could be identified that allowed a sufficient carbon supply for L-Phe production while at the same time an overflow metabolism leading to acetate by-product formation was avoided. The process approach is suitable for other production strains not only in lab-scale but also in pilot-scale bioreactors. |
| Links |
PubMed Online version:10.1002/bit.10428 |
| Keywords |
Bioreactors; Cells, Cultured; Escherichia coli/classification; Escherichia coli/genetics; Escherichia coli/metabolism; Feedback; Glucose/metabolism; Homeostasis/physiology; Phenylalanine/biosynthesis; Pilot Projects; Quality Control; Recombinant Proteins/biosynthesis; Recombination, Genetic; Reproducibility of Results; Sensitivity and Specificity; Species Specificity; Tyrosine/metabolism |
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