PMID:12399471

Citation	Schnick, C, Körtgen, N and Groth, G (2002) Complete inhibition of the tentoxin-resistant F1-ATPase from Escherichia coli by the phytopathogenic inhibitor tentoxin after substitution of critical residues in the alpha - and beta -subunit. J. Biol. Chem. 277:51003-7
Abstract	Substitution of critical residues in the alpha- and beta-subunit can turn the typically resistant ATP synthase from the bacterium Escherichia coli into an enzyme showing high sensitivity to the phytopathogenic inhibitor tentoxin, which usually affects only certain sensitive plant species. In contrast to recent results obtained with the thermophilic F(1) (Groth, G., Hisabori, T., Lill, H., and Bald, D. (2002) J. Biol. Chem. 277, 20117-20119), substitution of a critical serine in the beta-subunit (betaSer(59)), which is supposed to provide an important intermolecular hydrogen bond in the binding site, was not sufficient on its own for conferring tentoxin sensitivity to the E. coli F(1) complex. Superimposition of the chloroplast F(1)-tentoxin inhibitor complex on a homology model of the E. coli F(1) complex provided detailed information on the critical residues in the alpha-subunit of the binding cleft and allowed us to model the binding site according to the steric requirements of the inhibitor. Substitution of the highly conserved residue alphaLeu(64) seems to be most important for allowing access of the inhibitor to the binding site. Combining this substitution with either additional replacements in the alpha-subunit (Q49A, L95A, E96Q, I273M) or the replacement of Ser(59) in the beta-subunit enhanced the sensitivity to the inhibitor and resulted in a complete inhibition of the E. coli F(1)-ATPase by the plant-specific inhibitor tentoxin.
Links	PubMed Online version:10.1074/jbc.M206095200
Keywords	Amino Acid Sequence; Amino Acid Substitution; Base Sequence; DNA Primers; Escherichia coli/enzymology; Hydrogen Bonding; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mycotoxins/pharmacology; Peptides, Cyclic/chemistry; Peptides, Cyclic/pharmacology; Polymerase Chain Reaction; Protein Conformation; Protein Subunits; Proton-Translocating ATPases/antagonists & inhibitors; Proton-Translocating ATPases/chemistry; Sequence Alignment; Sequence Homology, Amino Acid; Serine
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.