PMID:12392714
| Citation |
Benov, L and Fridovich, I (2002) Induction of the soxRS regulon of Escherichia coli by glycolaldehyde. Arch. Biochem. Biophys. 407:45-8 |
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| Abstract |
The ability of short-chain sugars to cause oxidative stress has been examined using glycolaldehyde as the simplest sugar. Short-chain sugars autoxidize in air, producing superoxide and alpha,beta-dicarbonyls. In Escherichia coli the soxRS regulon mediates an oxidative stress response, which protects the cell against both superoxide-generating agents and nitric oxide. In superoxide dismutase-deficient E. coli mutants, glycolaldehyde induces fumarase C and nitroreductase A, which are regulated as members of the soxRS regulon. A mutational defect in soxRS eliminates that induction. This establishes that glycolaldehyde can cause induction of this defensive regulon. This effect of glycolaldehyde was oxygen-dependent, was not shown by glyoxal, and was not seen in the superoxide dismutase-replete parental strain, and it was abolished by a cell-permeable SOD mimetic. All of these suggest that superoxide radicals produced by the oxidation of glycolaldehyde played a key role in the induction. |
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| Keywords |
Acetaldehyde/analogs & derivatives; Acetaldehyde/pharmacology; Bacterial Proteins/drug effects; Bacterial Proteins/genetics; Drug Resistance, Neoplasm; Enzyme Activation/drug effects; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/drug effects; Escherichia coli Proteins/genetics; Fumarate Hydratase/drug effects; Fumarate Hydratase/genetics; Fumarate Hydratase/metabolism; Glucosephosphate Dehydrogenase/antagonists & inhibitors; Glucosephosphate Dehydrogenase/drug effects; Glucosephosphate Dehydrogenase/metabolism; Glyoxal/metabolism; Glyoxal/pharmacology; Mutation; Nitroreductases/drug effects; Nitroreductases/genetics; Nitroreductases/metabolism; Oxidative Stress; Oxygen/metabolism; Regulon/drug effects; Superoxide Dismutase/genetics; Superoxide Dismutase/metabolism; Trans-Activators/drug effects; Trans-Activators/genetics; Transcription Factors/drug effects; Transcription Factors/genetics |
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