PMID:12375104
| Citation |
Cook, GM, Cruz-Ramos, H, Moir, AJ and Poole, RK (2002) A novel haem compound accumulated in Escherichia coli overexpressing the cydDC operon, encoding an ABC-type transporter required for cytochrome assembly. Arch. Microbiol. 178:358-69 |
|---|---|
| Abstract |
cydDC genes encode a heterodimeric ABC transporter required for assembly of the membrane-bound cytochrome bd quinol oxidase and periplasmic cytochromes. Here, we demonstrate that overexpression of functional cydDC genes on a multicopy plasmid results in elevated levels of cytochromes b and d, but most notably formation in anaerobically grown cells of a novel haem-containing component P-574. The pigment has a distinctive absorbance at 574-579 nm and 448 nm in reduced minus oxidised spectra and renders over-producing cells reddish in colour. The highest levels of P-574 were observed in mutants (cydAB) in the structural genes for the polypeptides of cytochrome bd. P-574 is labile; its spectral signal is reduced in cells that are frozen-thawed or subjected to mechanical disruption. P-574 was not detected in cytoplasmic or periplasmic fractions and was predominantly associated with the cell membrane. P-574 did not bind CO or cyanide. Production of P-574 was dependent on haem biosynthesis indicating that it is a haem-containing molecule or derived from haem biosynthesis. These findings suggest that P-574 may result from association of a haem compound with overexpressed transporter subunits, but not with oxidase subunits, and are consistent with an intimate link between the transporter and haem processing during oxidase assembly. |
| Links |
PubMed Online version:10.1007/s00203-002-0467-6 |
| Keywords |
ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Biological Transport/genetics; Cytochromes/biosynthesis; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Heme/biosynthesis; Heme/metabolism; Operon; Oxidoreductases/biosynthesis; Plasmids/genetics |
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